1. Blocking Visualization of proteins in membranes (non-compulsory): As an non-compulsory step, we will confirm the protein had been transferred efficiently by staining the membrane with ponceau purple. Incubate the membrane in ponceau for five minutes and wash with water till the...
Western Transfer, also called Western Blotting, is a fast immunoblotting method for figuring out the presence of a specific protein in a fancy combination of proteins similar to cell lysates or sera. The method exploits each the effectivity of SDS-PAGE to...
Publication in peer-reviewed journals is an important step in the scientific course of. However, publication will not be merely the reporting of info arising from an easy evaluation thereof. Authors have broad latitude when writing their reviews and could also be...
Recently, NIH has funded a middle for autophagy analysis named the Autophagy, Inflammation, and Metabolism (AIM) Center of Biomedical Research Excellence, situated at the University of New Mexico Health Science Center (UNM HSC), with aspirations to advertise autophagy analysis regionally, nationally,...
As the tempo of biomedical innovation quickly evolves, there’s a want to coach researchers to know regulatory science challenges related to medical translation. We describe a pilot course aimed toward addressing this want delivered collectively by means of the Mayo Clinic...
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The anti-androgenic effect of chronic exposure to semicarbazide on male Japanese flounder (Paralichthys olivaceus) and its potential mechanisms. Journal: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP September/23/2018 Semicarbazide (SMC), a new marine pollutant, has anti-estrogenic effects on female Japanese...
Estradiol 17α-dehydrogenase publication: Renwick, A.G.C. and Engel, L.L. The partial purification of 17α- and 17β-estradiol dehydrogenase activities from chicken liver. Biochim. Biophys. Acta 146 (1967) 336–348. I would apprecitate any, even unpublished or indirect information about this enzyme. (EC 1.1.1.148) 17β-Hydroxysteroid...