Binding of naturally occurring human anti-DNA autoantibodies to beta-estradiol and native DNA has been investigated. Sera from seven SLE sufferers had been examined for his or her reactivity with beta-estradiol and native calf thymus DNA. Solid Phase enzyme immunoassay was carried...
Subtle modulation of antibody-binding properties by protein engineering usually lies with an correct structural and energetic description of how an antigen is recognised. Thus, with the intent to extend the affinity and add a bias in favour of pure estradiol in...
This week we will talk about cholesterol, the molecule that causes so much damage in the population, and how gene therapy, the use of genes as a therapeutic tool, may be the great solution that we were all waiting to face...
1. Blocking Visualization of proteins in membranes (non-compulsory): As an non-compulsory step, we will confirm the protein had been transferred efficiently by staining the membrane with ponceau purple. Incubate the membrane in ponceau for five minutes and wash with water till the...
Western Transfer, also called Western Blotting, is a fast immunoblotting method for figuring out the presence of a specific protein in a fancy combination of proteins similar to cell lysates or sera. The method exploits each the effectivity of SDS-PAGE to...
VPS35 Promotion Dates: March 1st– April 30th, 2020 StressMarq is pleased to offer 20% off our retail price on VPS35 Antibodies, for a total of 50% off our retail price, AND an extra 25% off our VPS35 ELISA Kit. Filtered VPS35...
The anti-androgenic effect of chronic exposure to semicarbazide on male Japanese flounder (Paralichthys olivaceus) and its potential mechanisms. Journal: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP September/23/2018 Semicarbazide (SMC), a new marine pollutant, has anti-estrogenic effects on female Japanese...
Estradiol 17α-dehydrogenase publication: Renwick, A.G.C. and Engel, L.L. The partial purification of 17α- and 17β-estradiol dehydrogenase activities from chicken liver. Biochim. Biophys. Acta 146 (1967) 336–348. I would apprecitate any, even unpublished or indirect information about this enzyme. (EC 1.1.1.148) 17β-Hydroxysteroid...
