Highly specific anti-estradiol antibodies: structural characterisation and binding diversity.
March 30, 2021
array, Assay, Autophagy, Inflammation, and Metabolism (AIM) Center of Biomedical Research Excellence: supporting the next generation of autophagy researchers and fostering international collaborations., Gerbil, Goat, Guinea Pig, Misrepresentation and distortion of research in biomedical literature., My Blog, profiling, Pure, purified, Rabbit, Real-time, Recombinant, reverse, RIA, rnai, Translating innovation in biomedical research: Design and delivery of a competency-based regulatory science course.
0 Comments
Subtle modulation of antibody-binding properties by protein engineering usually lies with an correct structural and energetic description of how an antigen is recognised. Thus, with the intent to extend the affinity and add a bias in favour of pure estradiol in contrast with its chemically modified immunogen, we have now decided the crystal construction of two anti-estradiol monoclonal antibodies, 10G6D6 and 17E12E5. Although generated towards the identical estradiol by-product, these antibodies share little sequence id, which is mirrored in dissimilar binding pockets and in several positioning of the steroid.
In each antibodies the attribute 17-hydroxyl group is buried deeply on the backside of hydrophobic pockets and stabilised by hydrogen bonds. Apart from this similarity, the steroid is oriented in a different way within the respective binding pockets. The excessive specificity of each antibodies has been mapped out, and even carefully associated steroids present low cross-reactivity. The structural research of the advanced shaped between 10G6D6 and 6-CMO-estradiol have recognized contacts between the 6-CMO coupling linker and an arginine residue from the heavy chain CDR2 phase.
Functional characterization of two anti-estradiol antibodies as deduced from modelling and site-directed mutagenesis experiments.
Monoclonal antibodies are actually broadly used to measure the focus of steroid hormones in human serum samples. The nice improvement of molecular engineering methods over the previous 10 years has made doable the advance of specificity and/or sensitivity of chosen antibodies. We have obtained two monoclonal antibodies, 17E12E5 and 10G6D6, utilizing estradiol-6-ethyl methoxy carbonyl (EMC)-bovine serum albumin (BSA) as immunogen. To tentatively enhance their affinities for pure estradiol, we have now initiated their structural and practical research.
For this goal, we have now cloned and sequenced the genes encoding the variable fragments of every antibody. Single chain variable fragments (scFv) had been produced into the periplasmic house of E. coli utilizing the pLIP6 expression vector. Mapping of the practical buildings of each antibodies was obtained by mixture of modelling and mutational analyses along with cross-reaction research. The two binding pockets are described and fashions of estradiol complexed to 17E12E5 and 10G6D6 are proposed.
Differential impact of estradiol on antibody secretion of murine hybridomas.
The want for elevated antibody manufacturing by hybridomas has been approached by the addition to cell cultures of various progress components; in vitro addition of estradiol-17beta (E2) to human blood lymphocytes will increase the buildup of plasma-blasts and Ig-secreting cells. Four totally different murine-murine hybridomas secreting totally different monoclonal antibodies (MAbs) had been handled with E2. Specific antibody focus was measured by enzyme-linked immunoadsorbent assay (ELISA) in tradition supernatants whereas expression of E2-receptor within the hybridoma cells was decided by polymerase chain response (PCR).
When E2 was added as a progress complement to alpha-estrogen receptor constructive murine-murine hybridomas it enhanced MAb secretion by as a lot as 255%, in a dose-dependant method. This impact lasted for so long as the alpha-estrogen receptor was detected within the hybridoma cells, was inhibited by tamoxifen and was not noticed in alpha-estrogen receptor detrimental hybridomas. The artificial estrogen analogue diethylstilbestrol had no impact. Estradiol-17beta needs to be added to the checklist of hybridoma-inducing progress components.
Expanding the conformational range by random insertions to CDRH2 leads to improved anti-estradiol antibodies.
The size of the heavy chain complementarity-determining area 2 (CDRH2) was prolonged past what’s present in germline genes to enhance the binding properties of an anti-estradiol antibody. The earlier immunochemical characterization and the molecular modeling of the excessive affinity (Ka=3.9×10(8)) murine anti-estradiol antibody 57-2 urged that part of the antigen was loosely acknowledged by the antibody. The CDRH2, due to its shut location however scarce contacts with the hapten, was thought of as a conceivable goal for mutagenesis. Libraries with both two, three or 4 random amino acid insertions within the tip of the CDRH2 loop had been constructed and displayed on the M13 filamentous phage as Fab fragments.
Mutations had been launched additionally into the remainder of the VHdomain by error-prone polymerase chain response to permit the encircling buildings to adapt to the prolonged CDRH2. After the panning of the libraries with an antigen off-rate-based choice, a variety of energetic clones, most of which confirmed considerably improved affinity and specificity, had been remoted, characterised and sequenced. They additionally counsel that the repertoire of antibody libraries might be expanded by extending the size of the CDR loops past that naturally offered by the given set of germline genes. This type of mutagenesis might be usually helpful for the engineering of hapten-binding antibodies.
Subcellular localization of estradiol receptor in MCF7 cells studied with nanogold-labelled antibody fragments.
Ultrastructural localization research of estradiol receptor in hormone-deprived and hormone-stimulated MCF7 cells had been performed utilizing F(ab’) fragments of three totally different antibodies (#402, 13H2, HT277) covalently linked to nanogold. These ultra-small, non-charged immunoreagents, mixed with a size-enlargement by silver enhancement, localized estradiol receptor in each nuclear and cytoplasmic areas of non-stimulated goal cells; stimulation with the steroid induced a predominantly nuclear labelling.
In the cytoplasm of resting cells, tagging was usually noticed at or within the proximity of stress fibers. In the nucleus a big proportion of receptor was discovered contained in the nucleolus, specifically with the reagent derived from antibody 13H2. We postulate that totally different accessibilities of receptor epitopes account for the totally different labelling densities noticed at cytoskeletal components and the nucleoli. This phase is now being focused by random mutagenesis to pick mutants with a choice for pure estradiol in comparison with the branched hapten.
Cross-linking of 17 beta-estradiol to monoclonal antibodies by direct UV irradiation: software to an enzyme immunometric assay.
Ultraviolet irradiation was used to cross-link 17 beta-estradiol on to monoclonal anti-17 beta-estradiol antibodies coated on 96-well microtiter plates. Cross-linking effectivity was immediately correlated with each irradiation power and wave-length. The greatest outcomes had been obtained at 254 (10 J/cm2, 45-min irradiation) and 312 nm (40 J/cm2, 160-min irradiation). The outcomes point out that the construction of the antibody can tolerate a variety of totally different insertions within the CDRH2 area.
The irradiation totally denatured each particular person molecules (i.e., 17 beta-estradiol and monoclonal anti-17 beta-estradiol antibody), however 17 beta-estradiol was at the least partly protected when immunologically certain to the paratope of the antibody. Four totally different monoclonal anti-17 beta-estradiol antibodies yielded constructive outcomes, demonstrating that this photo-cross-linking has appreciable potential.
 isa cadmium |
|||
| ISA22 | Consort | ea | EUR 91 |
 cadmium electrode |
|||
| ISE22B | Consort | ea | EUR 352 |
 cadmium orthophosphate |
|||
| 20-abx186402 | Abbexa |
|
|
|
|||
 Atazanavir sulphate |
|||
| GP7849-100MG | Glentham Life Sciences | 100 mg | EUR 86 |
Atazanavir sulphate |
|||
| GP7849-1G | Glentham Life Sciences | 1 g | EUR 221 |
Atazanavir sulphate |
|||
| GP7849-250MG | Glentham Life Sciences | 250 mg | EUR 126 |
 Tobramycin sulphate |
|||
| GA2734-100MG | Glentham Life Sciences | 100 mg | EUR 70 |
Tobramycin sulphate |
|||
| GA2734-1G | Glentham Life Sciences | 1 g | EUR 229 |
Tobramycin sulphate |
|||
| GA2734-250MG | Glentham Life Sciences | 250 mg | EUR 110 |
 Capastat sulphate |
|||
| GA2874-1G | Glentham Life Sciences | 1 g | EUR 134 |
Capastat sulphate |
|||
| GA2874-5G | Glentham Life Sciences | 5 g | EUR 365 |
 Minoxidil Sulphate |
|||
| abx184735-1g | Abbexa | 1 g | EUR 787 |
|
|||
 Copper Sulphate |
|||
| abx186748-5g | Abbexa | 5 g | EUR 189 |
|
|||
 DHEA Sulphate antibody |
|||
| 20-1424 | Fitzgerald | 100 ul | EUR 657 |
|
Description: Sheep polyclonal DHEA Sulphate antibody |
|||
) G418 SULPHATE (POWDER) |
|||
| 61-234-RG | CORNING | 5 g/pk | EUR 179 |
|
Description: Media Catalog; Cell Culture Reagents |
|||
) Cefadroxil (hydrate) |
|||
| C3991-1000 | ApexBio | 1 g | EUR 428 |
|
Description: Cefadroxil is a new semisynthetic cephalosporin with a broad antibacterial spectrum and a high chemotherapeutic potential [1]. In vitro: The inhibitory activity of this compound was similar to that of cephalexin and cephradine when tested against 602 clinical isolates on Mueller-Hinton medium. |
|||
Cefadroxil (hydrate) |
|||
| C3991-250 | ApexBio | 250 mg | EUR 163 |
|
Description: Cefadroxil is a new semisynthetic cephalosporin with a broad antibacterial spectrum and a high chemotherapeutic potential [1]. In vitro: The inhibitory activity of this compound was similar to that of cephalexin and cephradine when tested against 602 clinical isolates on Mueller-Hinton medium. |
|||
Cefadroxil (hydrate) |
|||
| C3991-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 113 |
|
Description: Cefadroxil is a new semisynthetic cephalosporin with a broad antibacterial spectrum and a high chemotherapeutic potential [1]. In vitro: The inhibitory activity of this compound was similar to that of cephalexin and cephradine when tested against 602 clinical isolates on Mueller-Hinton medium. |
|||
Cefadroxil (hydrate) |
|||
| C3991-500 | ApexBio | 500 mg | EUR 263 |
|
Description: Cefadroxil is a new semisynthetic cephalosporin with a broad antibacterial spectrum and a high chemotherapeutic potential [1]. In vitro: The inhibitory activity of this compound was similar to that of cephalexin and cephradine when tested against 602 clinical isolates on Mueller-Hinton medium. |
|||
) FR122047 (hydrate) |
|||
| C5551-10 | ApexBio | 10 mg | EUR 206 |
|
Description: FR122047 is a selective inhibitor of cyclooxygenase (COX)-1 [1].Cyclooxygenase (COX)-1 is constitutively expressed in almost all tissues. COX-1 gene has been considered to be a ?housekeeping? gene. |
|||
FR122047 (hydrate) |
|||
| C5551-5 | ApexBio | 5 mg | EUR 137 |
|
Description: FR122047 is a selective inhibitor of cyclooxygenase (COX)-1 [1].Cyclooxygenase (COX)-1 is constitutively expressed in almost all tissues. COX-1 gene has been considered to be a ?housekeeping? gene. |
|||
FR122047 (hydrate) |
|||
| C5551-50 | ApexBio | 50 mg | EUR 731 |
|
Description: FR122047 is a selective inhibitor of cyclooxygenase (COX)-1 [1].Cyclooxygenase (COX)-1 is constitutively expressed in almost all tissues. COX-1 gene has been considered to be a ?housekeeping? gene. |
|||
 Amikacin hydrate |
|||
| B1657-50 | ApexBio | 50 mg | EUR 186 |
|
Description: Amikacin is an aminoglycoside antibiotic used to treat different types of bacterial infections. |
|||
 Cefprozil hydrate |
|||
| B2142-10 | ApexBio | 10 mg | EUR 309 |
|
Description: Cefprozil hydrate |
|||
Cefprozil hydrate |
|||
| B2142-5 | ApexBio | 5 mg | EUR 187 |
|
Description: Cefprozil hydrate |
|||
Cefprozil hydrate |
|||
| B2142-50 | ApexBio | 50 mg | EUR 837 |
|
Description: Cefprozil hydrate |
|||
 Sitafloxacin Hydrate |
|||
| B2150-10 | ApexBio | 10 mg | EUR 309 |
|
Description: Sitafloxacin Hydrate |
|||
Sitafloxacin Hydrate |
|||
| B2150-5 | ApexBio | 5 mg | EUR 187 |
|
Description: Sitafloxacin Hydrate |
|||
Sitafloxacin Hydrate |
|||
| B2150-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 247 |
|
Description: Sitafloxacin Hydrate |
|||
Sitafloxacin Hydrate |
|||
| B2150-50 | ApexBio | 50 mg | EUR 837 |
|
Description: Sitafloxacin Hydrate |
|||
Sitafloxacin Hydrate |
|||
| B2150-S | ApexBio | Evaluation Sample | EUR 81 |
|
Description: Sitafloxacin Hydrate |
|||
) Methylstat (hydrate) |
|||
| B2836-1 | Biovision | EUR 142 | |
Methylstat (hydrate) |
|||
| B2836-5 | Biovision | EUR 370 | |
 Felbamate hydrate |
|||
| B1145-10 | ApexBio | 10 mg | EUR 139 |
|
Description: Felbamate hydrate (FBM) is a potent nonsedative anticonvulsant whose clinical effect may be related to the inhibition of N-methyl-D-aspartate (NMDA) . |
|||
Felbamate hydrate |
|||
| B1145-50 | ApexBio | 50 mg | EUR 357 |
|
Description: Felbamate hydrate (FBM) is a potent nonsedative anticonvulsant whose clinical effect may be related to the inhibition of N-methyl-D-aspartate (NMDA) . |
|||
 Posaconazole hydrate |
|||
| B1225-10 | ApexBio | 10 mg | EUR 215 |
|
Description: Posaconazole is a broad-spectrum, second generation, triazole compound with antifungal activity. Posaconazole strongly inhibits 14-alpha demethylase, a cytochrome P450-dependent enzyme |
|||
Posaconazole hydrate |
|||
| B1225-5 | ApexBio | 5 mg | EUR 192 |
|
Description: Posaconazole is a broad-spectrum, second generation, triazole compound with antifungal activity. Posaconazole strongly inhibits 14-alpha demethylase, a cytochrome P450-dependent enzyme |
|||
 Fluconazole hydrate |
|||
| B1226-100 | ApexBio | 100 mg | EUR 96 |
|
Description: Fluconazole (hydrate) is a triazole antifungal drug used in the treatment and prevention of superficial and systemic fungal infections. |
|||
Fluconazole hydrate |
|||
| B1226-1000 | ApexBio | 1 g | EUR 108 |
|
Description: Fluconazole (hydrate) is a triazole antifungal drug used in the treatment and prevention of superficial and systemic fungal infections. |
|||
Fluconazole hydrate |
|||
| B1226-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 108 |
|
Description: Fluconazole (hydrate) is a triazole antifungal drug used in the treatment and prevention of superficial and systemic fungal infections. |
|||
Fluconazole hydrate |
|||
| B1226-5000 | ApexBio | 5 g | EUR 224 |
|
Description: Fluconazole (hydrate) is a triazole antifungal drug used in the treatment and prevention of superficial and systemic fungal infections. |
|||
 Stevioside hydrate |
|||
| B1242-25 | Biovision | EUR 359 | |
Stevioside hydrate |
|||
| B1242-5 | Biovision | EUR 131 | |
 Tenofovir hydrate |
|||
| B1252-10 | ApexBio | 10 mg | EUR 155 |
|
Description: Tenofovir hydrate is an inhibitor of reverse transcriptase used for the treatment of the human immunodeficiency virus 1(HIV-1) and hepatitis B [1].Tenofovir hydrate is an antiviral pro-drug and the class of nucleoside reverse transcriptase inhibitor. |
|||
Tenofovir hydrate |
|||
| B1252-5 | ApexBio | 5 mg | EUR 125 |
|
Description: Tenofovir hydrate is an inhibitor of reverse transcriptase used for the treatment of the human immunodeficiency virus 1(HIV-1) and hepatitis B [1].Tenofovir hydrate is an antiviral pro-drug and the class of nucleoside reverse transcriptase inhibitor. |
|||
Tenofovir hydrate |
|||
| B1252-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 132 |
|
Description: Tenofovir hydrate is an inhibitor of reverse transcriptase used for the treatment of the human immunodeficiency virus 1(HIV-1) and hepatitis B [1].Tenofovir hydrate is an antiviral pro-drug and the class of nucleoside reverse transcriptase inhibitor. |
|||
Tenofovir hydrate |
|||
| B1252-50 | ApexBio | 50 mg | EUR 231 |
|
Description: Tenofovir hydrate is an inhibitor of reverse transcriptase used for the treatment of the human immunodeficiency virus 1(HIV-1) and hepatitis B [1].Tenofovir hydrate is an antiviral pro-drug and the class of nucleoside reverse transcriptase inhibitor. |
|||
 Bosentan Hydrate |
|||
| B1521-100 | ApexBio | 100 mg | EUR 212 |
|
Description: IC50: Inhibiting endothelin receptor A and B with an IC50 of 15.1 ± 1.6 ?M in P388/dx cells.A sulfonamide-derived, competitive and specific endothelin receptor antagonist with a relatively higher affinity to the endothelin A receptor than endothelin B receptor. |
|||
Bosentan Hydrate |
|||
| B1521-200 | ApexBio | 200 mg | EUR 305 |
|
Description: IC50: Inhibiting endothelin receptor A and B with an IC50 of 15.1 ± 1.6 ?M in P388/dx cells.A sulfonamide-derived, competitive and specific endothelin receptor antagonist with a relatively higher affinity to the endothelin A receptor than endothelin B receptor. |
|||
Bosentan Hydrate |
|||
| B1521-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 125 |
|
Description: IC50: Inhibiting endothelin receptor A and B with an IC50 of 15.1 ± 1.6 ?M in P388/dx cells.A sulfonamide-derived, competitive and specific endothelin receptor antagonist with a relatively higher affinity to the endothelin A receptor than endothelin B receptor. |
|||
Bosentan Hydrate |
|||
| B1521-50 | ApexBio | 50 mg | EUR 142 |
|
Description: IC50: Inhibiting endothelin receptor A and B with an IC50 of 15.1 ± 1.6 ?M in P388/dx cells.A sulfonamide-derived, competitive and specific endothelin receptor antagonist with a relatively higher affinity to the endothelin A receptor than endothelin B receptor. |
|||
Bosentan Hydrate |
|||
| B1521-500 | ApexBio | 500 mg | EUR 572 |
|
Description: IC50: Inhibiting endothelin receptor A and B with an IC50 of 15.1 ± 1.6 ?M in P388/dx cells.A sulfonamide-derived, competitive and specific endothelin receptor antagonist with a relatively higher affinity to the endothelin A receptor than endothelin B receptor. |
|||
 Cephalexin Hydrate |
|||
| B1522-25G | Biovision | EUR 327 | |
Cephalexin Hydrate |
|||
| B1522-5G | Biovision | EUR 120 | |
 Guanosine Hydrate |
|||
| B3678-200 | ApexBio | 200 mg | EUR 142 |
Cephalexin Hydrate |
|||
| C024-10G | TOKU-E | 10 g | EUR 208 |
Cephalexin Hydrate |
|||
| C024-25G | TOKU-E | 25 g | EUR 375 |
Cephalexin Hydrate |
|||
| C024-5G | TOKU-E | 5 g | EUR 132 |
) Tivozanib (hydrate) |
|||
| C3415-10 | ApexBio | 10 mg | EUR 216 |
|
Description: Tivozanib, also known as AV-951 and KRN-951, is an orally active, ATP-competitive, small-molecule, quinoline-urea derivative. Tivozanib is a pan-VEGFR tyrosine kinase inhibitor. |
|||
Tivozanib (hydrate) |
|||
| C3415-5 | ApexBio | 5 mg | EUR 174 |
|
Description: Tivozanib, also known as AV-951 and KRN-951, is an orally active, ATP-competitive, small-molecule, quinoline-urea derivative. Tivozanib is a pan-VEGFR tyrosine kinase inhibitor. |
|||
 Doripenem Hydrate |
|||
| E1KS1374 | EnoGene | 10mg | EUR 521 |
) Saxagliptin (hydrate) |
|||
| HY-10285A | MedChemExpress | 50mg | EUR 380 |
 Napsagatran hydrate |
|||
| HY-15759A | MedChemExpress | 10mg | EUR 11716 |
) Miriplatin (hydrate) |
|||
| HY-16325 | MedChemExpress | 100mg | EUR 946 |
) Posaconazole (hydrate) |
|||
| HY-17373A | MedChemExpress | 10mg | EUR 176 |
) Cyclophosphamide (hydrate) |
|||
| HY-17420A | MedChemExpress | 100mg | EUR 108 |
) Azithromycin (hydrate) |
|||
| HY-17506A | MedChemExpress | 100mg | EUR 160 |
) Bosentan (hydrate) |
|||
| HY-A0013A | MedChemExpress | 200mg | EUR 354 |
) Fluconazole (hydrate) |
|||
| HY-B0101A | MedChemExpress | 100mg | EUR 119 |
) Felbamate (hydrate) |
|||
| HY-B0184A | MedChemExpress | 10mg | EUR 119 |
) Gadodiamide (hydrate) |
|||
| HY-B0266 | MedChemExpress | 10mM/1mL | EUR 239 |
) Enoxacin (hydrate) |
|||
| HY-B0268A | MedChemExpress | 100mg | EUR 119 |
) Levofloxacin (hydrate) |
|||
| HY-B0330A | MedChemExpress | 100mg | EUR 119 |
) Sitafloxacin (hydrate) |
|||
| HY-B0395C | MedChemExpress | 10mg | EUR 187 |
) Amikacin (hydrate) |
|||
| HY-B0509 | MedChemExpress | 5g | EUR 349 |
) Terpin (hydrate) |
|||
| HY-B1063 | MedChemExpress | 1g | EUR 119 |
) Xanthopterin (hydrate) |
|||
| HY-119674A | MedChemExpress | 100mg | EUR 108 |
) Ivacaftor (hydrate) |
|||
| HY-13017B | MedChemExpress | 5mg | EUR 147 |
) Tofogliflozin (hydrate) |
|||
| HY-13413 | MedChemExpress | 5mg | EUR 165 |
) Tenofovir (hydrate) |
|||
| HY-13910A | MedChemExpress | 10mg | EUR 131 |
 Fanapanel hydrate |
|||
| HY-15069A | MedChemExpress | 10mM/1mL | EUR 263 |
 Grazoprevir hydrate |
|||
| HY-15298B | MedChemExpress | 10mg | EUR 243 |
 Pranlukast hydrate |
|||
| GP8160-250MG | Glentham Life Sciences | 250 mg | EUR 229 |
Pranlukast hydrate |
|||
| GP8160-25MG | Glentham Life Sciences | 25 mg | EUR 78 |
Pranlukast hydrate |
|||
| GP8160-50MG | Glentham Life Sciences | 50 mg | EUR 116 |
 Entecavir hydrate |
|||
| GP8732-100MG | Glentham Life Sciences | 100 mg | EUR 110 |
Entecavir hydrate |
|||
| GP8732-250MG | Glentham Life Sciences | 250 mg | EUR 190 |
 Dynasore hydrate |
|||
| GP9526-25MG | Glentham Life Sciences | 25 mg | EUR 252 |
Dynasore hydrate |
|||
| GP9526-5MG | Glentham Life Sciences | 5 mg | EUR 114 |
 Methylcobalamin hydrate |
|||
| GV9033-1G | Glentham Life Sciences | 1 g | EUR 94 |
Methylcobalamin hydrate |
|||
| GV9033-250MG | Glentham Life Sciences | 250 mg | EUR 58 |
Methylcobalamin hydrate |
|||
| GV9033-5G | Glentham Life Sciences | 5 g | EUR 229 |
 Tigecycline hydrate |
|||
| GK6202-100MG | Glentham Life Sciences | 100 mg | EUR 110 |
Tigecycline hydrate |
|||
| GK6202-25MG | Glentham Life Sciences | 25 mg | EUR 62 |
Tigecycline hydrate |
|||
| GK6202-5MG | Glentham Life Sciences | 5 mg | EUR 46 |
-Catechin hydrate) (+)-Catechin hydrate |
|||
| GL6828-10G | Glentham Life Sciences | 10 g | EUR 126 |
(+)-Catechin hydrate |
|||
| GL6828-1G | Glentham Life Sciences | 1 g | EUR 54 |
(+)-Catechin hydrate |
|||
| GL6828-5G | Glentham Life Sciences | 5 g | EUR 86 |
 Morin hydrate |
|||
| GL8475-1G | Glentham Life Sciences | 1 g | EUR 56 |
 Cidofovir hydrate |
|||
| GP0753-10MG | Glentham Life Sciences | 10 mg | EUR 74 |
 Gatifloxacin hydrate |
|||
| GP2111-250MG | Glentham Life Sciences | 250 mg | EUR 158 |
Gatifloxacin hydrate |
|||
| GP2111-50MG | Glentham Life Sciences | 50 mg | EUR 90 |
-Taxifolin hydrate) (+/-)-Taxifolin hydrate |
|||
| GP3540-100MG | Glentham Life Sciences | 100 mg | EUR 126 |
(+/-)-Taxifolin hydrate |
|||
| GP3540-25MG | Glentham Life Sciences | 25 mg | EUR 68 |
(+/-)-Taxifolin hydrate |
|||
| GP3540-500MG | Glentham Life Sciences | 500 mg | EUR 329 |
 Enalaprilat hydrate |
|||
| GP3972-100MG | Glentham Life Sciences | 100 mg | EUR 317 |
Enalaprilat hydrate |
|||
| GP3972-25MG | Glentham Life Sciences | 25 mg | EUR 142 |
Enalaprilat hydrate |
|||
| GP3972-50MG | Glentham Life Sciences | 50 mg | EUR 205 |
 Bosentan hydrate |
|||
| GP5893-100MG | Glentham Life Sciences | 100 mg | EUR 190 |
Bosentan hydrate |
|||
| GP5893-10MG | Glentham Life Sciences | 10 mg | EUR 86 |
Bosentan hydrate |
|||
| GP5893-50MG | Glentham Life Sciences | 50 mg | EUR 130 |
 Rebamipide hydrate |
|||
| GP6330-1G | Glentham Life Sciences | 1 g | EUR 70 |
Rebamipide hydrate |
|||
| GP6330-5G | Glentham Life Sciences | 5 g | EUR 134 |
 Nystatin hydrate |
|||
| GA3995-10G | Glentham Life Sciences | 10 g | EUR 86 |
Nystatin hydrate |
|||
| GA3995-1G | Glentham Life Sciences | 1 g | EUR 46 |
Nystatin hydrate |
|||
| GA3995-5G | Glentham Life Sciences | 5 g | EUR 62 |
 Ceftazidime hydrate |
|||
| GA6002-1G | Glentham Life Sciences | 1 g | EUR 91 |
 Methotrexate hydrate |
|||
| GA6211-1G | Glentham Life Sciences | 1 g | EUR 110 |
Methotrexate hydrate |
|||
| GA6211-5G | Glentham Life Sciences | 5 g | EUR 325 |
 Ceftibuten hydrate |
|||
| GA7818-100MG | Glentham Life Sciences | 100 mg | EUR 181 |
Ceftibuten hydrate |
|||
| GA7818-10MG | Glentham Life Sciences | 10 mg | EUR 70 |
Ceftibuten hydrate |
|||
| GA7818-25MG | Glentham Life Sciences | 25 mg | EUR 94 |
Ceftibuten hydrate |
|||
| GA7818-50MG | Glentham Life Sciences | 50 mg | EUR 120 |
Amikacin hydrate |
|||
| GA9944-5G | Glentham Life Sciences | 5 g | EUR 309 |
 Naringin hydrate |
|||
| GC2272-100G | Glentham Life Sciences | 100 g | EUR 110 |
Naringin hydrate |
|||
| GC2272-250G | Glentham Life Sciences | 250 g | EUR 181 |
Naringin hydrate |
|||
| GC2272-25G | Glentham Life Sciences | 25 g | EUR 62 |
 Esculin hydrate |
|||
| GC4131-100G | Glentham Life Sciences | 100 g | EUR 301 |
Esculin hydrate |
|||
| GC4131-10G | Glentham Life Sciences | 10 g | EUR 78 |
Esculin hydrate |
|||
| GC4131-25G | Glentham Life Sciences | 25 g | EUR 118 |
Esculin hydrate |
|||
| GC4131-50G | Glentham Life Sciences | 50 g | EUR 181 |
Esculin hydrate |
|||
| GC4131-5G | Glentham Life Sciences | 5 g | EUR 59 |
We used this authentic strategy to develop a brand new enzyme immunometric assay of 17 beta-estradiol based mostly on our beforehand described immunometric process, solid-phase immobilized epitope immunoassay, which makes use of chemical brokers to cross-link haptens by way of amino teams to specific antibodies. The assay was specific (no cross-reactivity with different pure steroids), exact, and delicate (detection restrict of 38 pg/mL in human serum). It correlated effectively with two aggressive industrial immunoassays when examined on 40 human sera.
Translating innovation in biomedical research: Design and delivery of a competency-based regulatory science course.
E
StressMarq
About Author
