This week we will talk about cholesterol, the molecule that causes so much damage in the population, and how gene therapy, the use of genes as a therapeutic tool, may be the great solution that we were all waiting to face...
1. Blocking Visualization of proteins in membranes (non-compulsory): As an non-compulsory step, we will confirm the protein had been transferred efficiently by staining the membrane with ponceau purple. Incubate the membrane in ponceau for five minutes and wash with water till the...
Western Transfer, also called Western Blotting, is a fast immunoblotting method for figuring out the presence of a specific protein in a fancy combination of proteins similar to cell lysates or sera. The method exploits each the effectivity of SDS-PAGE to...